Academic Journal
Cloning and expression of endo-β-glucanase II gene in Humicola insolens H31-3.
| Title: | Cloning and expression of endo-β-glucanase II gene in Humicola insolens H31-3. |
|---|---|
| Authors: | Tan, Huifang, Zhang, Guoqing, Zheng, Guangyu, Cui, Fumian, Qian, Shijun |
| Superior Title: | Frontiers of Biology in China; Sep2008, Vol. 3 Issue 3, p287-292, 6p |
| Abstract: | Endo-β-glucanase II (EG II) gene cDNA was isolated from the fungus Humicola insolens H31-3 by RT-PCR. It was cloned into the expression vector pGAPZαA. The resultant recombinant plasmid was introduced into Pichia pastoris GS115 by electroporation after being linearized by BspHI digestion. The recombinant Pichia pastoris strain was obtained and SDS-PAGE showed that the molecular weight of the expression protein was about 55 kD.The cultivation condition and the characteristics of the recombinant EG II were also explored. [ABSTRACT FROM AUTHOR] |
| Copyright of Frontiers of Biology in China is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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