Bibliographic Details
| Title: |
Polarised cell intercalation during Drosophila axis extension is robust to an orthogonal pull by the invaginating mesoderm. |
| Authors: |
Lye, Claire M.1 (AUTHOR) cmg38@cam.ac.uk, Blanchard, Guy B.1 (AUTHOR), Evans, Jenny1 (AUTHOR), Nestor-Bergmann, Alexander1 (AUTHOR), Sanson, Bénédicte1 (AUTHOR) cmg38@cam.ac.uk |
| Superior Title: |
PLoS Biology. 4/29/2024, Vol. 22 Issue 4, p1-31. 31p. |
| Subject Terms: |
*MESODERM, *ANIMAL development, *DROSOPHILA, *ECTODERM, *GASTRULATION, *MYOSIN |
| Abstract: |
As tissues grow and change shape during animal development, they physically pull and push on each other, and these mechanical interactions can be important for morphogenesis. During Drosophila gastrulation, mesoderm invagination temporally overlaps with the convergence and extension of the ectodermal germband; the latter is caused primarily by Myosin II–driven polarised cell intercalation. Here, we investigate the impact of mesoderm invagination on ectoderm extension, examining possible mechanical and mechanotransductive effects on Myosin II recruitment and polarised cell intercalation. We find that the germband ectoderm is deformed by the mesoderm pulling in the orthogonal direction to germband extension (GBE), showing mechanical coupling between these tissues. However, we do not find a significant change in Myosin II planar polarisation in response to mesoderm invagination, nor in the rate of junction shrinkage leading to neighbour exchange events. We conclude that the main cellular mechanism of axis extension, polarised cell intercalation, is robust to the mesoderm invagination pull. We find, however, that mesoderm invagination slows down the rate of anterior-posterior cell elongation that contributes to axis extension, counteracting the tension from the endoderm invagination, which pulls along the direction of GBE. Morphogenetic movements can generate forces that pull on adjacent tissues, which can in turn influence cell behaviors through mechanotransduction. Using computational analysis of gastrulating Drosophila embryos, this study shows that polarized cell intercalation is robust to the pull generated by mesoderm invagination, which however slows down the rate of anterior-posterior cell elongation. [ABSTRACT FROM AUTHOR] |
|
Copyright of PLoS Biology is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Premier |
|
Full text is not displayed to guests. |
Login for full access.
|