Bibliographic Details
| Title: |
RGS4 impacts carbohydrate and siderophore metabolism in Trichoderma reesei. |
| Authors: |
Schalamun, Miriam1 (AUTHOR), Molin, Eva Maria1 (AUTHOR), Schmoll, Monika1,2 (AUTHOR) monika.schmoll@univie.ac.at |
| Superior Title: |
BMC Genomics. 7/4/2023, Vol. 24 Issue 1, p1-17. 17p. |
| Subject Terms: |
*TRICHODERMA reesei, *CARBOHYDRATE metabolism, *PLANT cell walls, *BIOLOGICAL fitness, *CELLULASE, *SECONDARY metabolism, *G proteins, *GALACTOSE |
| Abstract: |
Background: Adaptation to complex, rapidly changing environments is crucial for evolutionary success of fungi. The heterotrimeric G-protein pathway belongs to the most important signaling cascades applied for this task. In Trichoderma reesei, enzyme production, growth and secondary metabolism are among the physiological traits influenced by the G-protein pathway in a light dependent manner. Results: Here, we investigated the function of the SNX/H-type regulator of G-protein signaling (RGS) protein RGS4 of T. reesei. We show that RGS4 is involved in regulation of cellulase production, growth, asexual development and oxidative stress response in darkness as well as in osmotic stress response in the presence of sodium chloride, particularly in light. Transcriptome analysis revealed regulation of several ribosomal genes, six genes mutated in RutC30 as well as several genes encoding transcription factors and transporters. Importantly, RGS4 positively regulates the siderophore cluster responsible for fusarinine C biosynthesis in light. The respective deletion mutant shows altered growth on nutrient sources related to siderophore production such as ornithine or proline in a BIOLOG phenotype microarray assay. Additionally, growth on storage carbohydrates as well as several intermediates of the D-galactose and D-arabinose catabolic pathway is decreased, predominantly in light. Conclusions: We conclude that RGS4 mainly operates in light and targets plant cell wall degradation, siderophore production and storage compound metabolism in T. reesei. [ABSTRACT FROM AUTHOR] |
|
Copyright of BMC Genomics is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Premier |
|
Full text is not displayed to guests. |
Login for full access.
|