Electronic Resource
Management of enzyme diversity in high-performance cellulolytic cocktails
| Title: | Management of enzyme diversity in high-performance cellulolytic cocktails |
|---|---|
| Publisher Information: | BioMed Central 2017 |
| Added Details: | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular European Union (UE). FP7 Ministerio de Economía y Competitividad (MINECO). España Reyes Sosa, Francisco Manuel López Morales, Macarena Platero Gómez, Ana Isabel Valbuena Crespo, Noelia Sánchez Zamorano, Laura Rocha Martín, Javier Molina Heredia, Fernando Publio |
| Document Type: | Electronic Resource |
| Abstract: | Background Modern biorefineries require enzymatic cocktails of improved efficiency to generate fermentable sugars from lignocellulosic biomass. Cellulolytic fungi, among other microorganisms, have demonstrated the highest potential in terms of enzymatic productivity, complexity and efficiency. On the other hand, under cellulolytic-inducing conditions, they often produce a considerable diversity of carbohydrate-active enzymes which allow them to adapt to changing environmental conditions. However, industrial conditions are fixed and adjusted to the optimum of the whole cocktail, resulting in underperformance of individual enzymes. Results One of these cellulolytic cocktails from Myceliophthora thermophila has been analyzed here by means of LC–MS/MS. Pure GH6 family members detected have been characterized, confirming previous studies, and added to whole cocktails to compare their contribution in the hydrolysis of industrial substrates. Finally, independent deletions of two GH6 family members, as an example of the enzymatic diversity management, led to the development of a strain producing a more efficient cellulolytic cocktail. Conclusions These data indicate that the deletion of noncontributive cellulases (here EG VI) can increase the cellulolytic efficiency of the cocktail, validating the management of cellulase diversity as a strategy to obtain improved fungal cellulolytic cocktails. |
| Index Terms: | Enzymatic hydrolysis, Cellulolytic cocktail improvement, Lignocellulosic biomass, Bioethanol, info:eu-repo/semantics/article |
| URL: | Biotechnology for Biofuels, 10 (156), 1-10. info:eu-repo/grantAgreement/MINECO/PCIN-2013-012-C02-02 info:eu-repo/grantAgreement/EC/FP7/604530 |
| Availability: | Open access content. Open access content http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
| Note: | English |
| Other Numbers: | SUE oai:idus.us.es:11441/63612 Reyes Sosa, F.M., López Morales, M., Platero Gómez, A.I., Valbuena Crespo, N., Sánchez Zamorano, L., Rocha Martín, J. y Molina Heredia, F.P. (2017). Management of enzyme diversity in high-performance cellulolytic cocktails. Biotechnology for Biofuels, 10 (156), 1-10. 1754-6834 10.1186/s13068-017-0845-6 1367137440 |
| Contributing Source: | UNIV DE SEVILLA From OAIster®, provided by the OCLC Cooperative. |
| Accession Number: | edsoai.on1367137440 |
| Database: | OAIster |
| Description not available. |