Academic Journal
Discrimination of Baphicacanthis Cusiae Rhizoma et radix and its adulterant species and establishment of an assay method for quality control
| Title: | Discrimination of Baphicacanthis Cusiae Rhizoma et radix and its adulterant species and establishment of an assay method for quality control |
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| Authors: | Yue Li, Qiong-Xi Yu, Lee-Fong Yau, Guo-Kai Huang, Jing-Guang Lu, Xiao-Xiao Liu, Zhi-Hong Jiang, Jing-Rong Wang |
| Superior Title: | Chinese Medicine, Vol 18, Iss 1, Pp 1-14 (2023) |
| Publisher Information: | BMC |
| Publication Year: | 2023 |
| Collection: | Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: | UHPLC Q-TOF-MS, Chromatographic fingerprint, Quantitative markers, Isatis indigotica Fort, 2-Benzoxazolinone, Acteoside, Other systems of medicine, RZ201-999 |
| Description: | Background Baphicacanthis Cusiae Rhizoma et Radix, commonly known as Nan-Ban-Lan-Gen (NBLG), is an essential traditional Chinese medicine that possesses diverse bioactivities, particularly noteworthy for its antiviral properties. Although NBLG has been listed in the Chinese Pharmacopoeia as an independent Chinese medicine, the establishment of a comprehensive quality standard for NBLG remains elusive. The absence of assay for marker compound in its quality standards has led to the lack of corresponding quality control measures for NBLG-containing preparations, and its discrimination from adulterant species in the market which thereby can significantly impact the efficacy and safety of NBLG-containing products. Methods Ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF-MS) was employed for comprehensive profiling of the chemical constituents of NBLG, the stem of Baphicacanthus cusia (Nees) Bremek (NBLJ), and the roots of Isatis indigotica Fort. (Bei-Ban-Lan-Gen, BBLG). Additionally, multivariate analysis was conducted to compare the chemical components of NBLG with those of NBLJ and BBLG. Furthermore, we established an optimized and validated HPLC method to obtain the fingerprint of NBLG and quantify the content of 2-benzoxazolinone and acteoside in the samples. Results A total of 73 compounds belonging to six classes were assigned in NBLG, with alkaloids being the most abundant and diverse species. High compositional similarities with significant differences in content were observed between NBLG and NBLJ. Moreover, the chemical profile of BBLG markedly differed from that of NBLG. An informative high performance liquid chromatography (HPLC) fingerprint of NBLG comprising seven characteristic peaks that can be used for quality assessment was established. Notably, we propose a quality control standard for NBLG, stipulating that the limit of content in dry weight for both 2-benzoxazolinone and acteoside should not be less than 0.010%. ... |
| Document Type: | article in journal/newspaper |
| Language: | English |
| ISSN: | 1749-8546 |
| Relation: | https://doi.org/10.1186/s13020-023-00777-x; https://doaj.org/toc/1749-8546; https://doaj.org/article/7ed6bca691e3422690e2009659ec9bf4 |
| DOI: | 10.1186/s13020-023-00777-x |
| Availability: | https://doi.org/10.1186/s13020-023-00777-x https://doaj.org/article/7ed6bca691e3422690e2009659ec9bf4 |
| Accession Number: | edsbas.F4B4184 |
| Database: | BASE |
| Description not available. |