Academic Journal
16S microbiome analysis of microbial communities in distribution centers handling fresh produce
| Title: | 16S microbiome analysis of microbial communities in distribution centers handling fresh produce |
|---|---|
| Authors: | Townsend, Anna, den Bakker, Hendrik C., Mann, Amy, Murphy, Claire M., Strawn, Laura K., Dunn, Laurel L. |
| Superior Title: | Front Microbiol |
| Publisher Information: | Frontiers Media S.A. |
| Publication Year: | 2023 |
| Collection: | PubMed Central (PMC) |
| Subject Terms: | Microbiology |
| Description: | Little is known about the microbial communities found in distribution centers (DCs), especially in those storing and handling food. As many foodborne bacteria are known to establish residence in food facilities, it is reasonable to assume that DCs handling foods are also susceptible to pathogen colonization. To investigate the microbial communities within DCs, 16S amplicon sequencing was completed on 317 environmental surface sponge swabs collected in DCs (n = 18) across the United States. An additional 317 swabs were collected in parallel to determine if any viable Listeria species were also present at each sampling site. There were significant differences in median diversity measures (observed, Shannon, and Chao1) across individual DCs, and top genera across all reads were Carnobacterium_A, Psychrobacter, Pseudomonas_E, Leaf454, and Staphylococcus based on taxonomic classifications using the Genome Taxonomy Database. Of the 39 16S samples containing Listeria ASVs, four of these samples had corresponding Listeria positive microbiological samples. Data indicated a predominance of ASVs identified as cold-tolerant bacteria in environmental samples collected in DCs. Differential abundance analysis identified Carnobacterium_A, Psychrobacter, and Pseudomonas_E present at a significantly greater abundance in Listeria positive microbiological compared to those negative for Listeria. Additionally, microbiome composition varied significantly across groupings within variables (e.g., DC, season, general sampling location). |
| Document Type: | text |
| Language: | English |
| Relation: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369000/; http://dx.doi.org/10.3389/fmicb.2023.1041936 |
| DOI: | 10.3389/fmicb.2023.1041936 |
| Availability: | https://doi.org/10.3389/fmicb.2023.1041936 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10369000/ |
| Rights: | Copyright © 2023 Townsend, den Bakker, Mann, Murphy, Strawn and Dunn. ; https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| Accession Number: | edsbas.F2C108C5 |
| Database: | BASE |
| Description not available. |