Academic Journal
A fluidic platform for mobility evaluation of zebrafish with gene deficiency
| Title: | A fluidic platform for mobility evaluation of zebrafish with gene deficiency |
|---|---|
| Authors: | Jia, Xiaoyu, Feng, Yibo, Ma, Wenju, Zhao, Wei, Liu, Yanan, Jing, Guangyin, Tian, Jing, Yang, Tao, Zhang, Ce |
| Superior Title: | Front Mol Neurosci |
| Publisher Information: | Frontiers Media S.A. |
| Publication Year: | 2023 |
| Collection: | PubMed Central (PMC) |
| Subject Terms: | Molecular Neuroscience |
| Description: | INTRODUCTION: Zebrafish is a suitable animal model for molecular genetic tests and drug discovery due to its characteristics including optical transparency, genetic manipulability, genetic similarity to humans, and cost-effectiveness. Mobility of the zebrafish reflects pathological conditions leading to brain disorders, disrupted motor functions, and sensitivity to environmental challenges. However, it remains technologically challenging to quantitively assess zebrafish's mobility in a flowing environment and simultaneously monitor cellular behavior in vivo. METHODS: We herein developed a facile fluidic device using mechanical vibration to controllably generate various flow patterns in a droplet housing single zebrafish, which mimics its dynamically flowing habitats. RESULTS: We observe that in the four recirculating flow patterns, there are two equilibrium stagnation positions for zebrafish constrained in the droplet, i.e., the “source” with the outward flow and the “sink” with the inward flow. Wild-type zebrafish, whose mobility remains intact, tend to swim against the flow and fight to stay at the source point. A slight deviation from streamline leads to an increased torque pushing the zebrafish further away, whereas zebrafish with motor neuron dysfunction caused by lipin-1 deficiency are forced to stay in the “sink,” where both their head and tail align with the flow direction. Deviation angle from the source point can, therefore, be used to quantify the mobility of zebrafish under flowing environmental conditions. Moreover, in a droplet of comparable size, single zebrafish can be effectively restrained for high-resolution imaging. CONCLUSION: Using the proposed methodology, zebrafish mobility reflecting pathological symptoms can be quantitively investigated and directly linked to cellular behavior in vivo. |
| Document Type: | text |
| Language: | English |
| Relation: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117665/; http://dx.doi.org/10.3389/fnmol.2023.1114928 |
| DOI: | 10.3389/fnmol.2023.1114928 |
| Availability: | https://doi.org/10.3389/fnmol.2023.1114928 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117665/ |
| Rights: | Copyright © 2023 Jia, Feng, Ma, Zhao, Liu, Jing, Tian, Yang and Zhang. ; https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| Accession Number: | edsbas.CF4B2D56 |
| Database: | BASE |
| Description not available. |