Academic Journal
Vinyl chloride-induced DNA adducts I: Quantitative determination of N2,3-ethenoguanine based on electrophore labeling
| Title: | Vinyl chloride-induced DNA adducts I: Quantitative determination of N2,3-ethenoguanine based on electrophore labeling |
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| Authors: | Fedtke, N., Boucheron, J.A., Turner, M. J., Swenberg, J. A. |
| Publisher Information: | Oxford University Press |
| Publication Year: | 1990 |
| Collection: | HighWire Press (Stanford University) |
| Subject Terms: | ORIGINAL ARTICLES |
| Description: | A sensitive assay for quantitative determination of the vinyl chloride (VC)-induced cyclic DNA adduct N2,3-ethenoguanine (EG) was developed. The method is based on the detection of EG as its di-pentafluorobenzyl derivative (3,5-PFB 2 -EG) This compound exhibited good gas chromatographic properties and was detected with high sensitivity by gas chromatography with electron capture detection (limit of detection 300 amol/µl injected solution) or with negative ion chemical ionization mass spectrometry monitoring the [M-181]− fragment ion at m/z 354 (GC-NICI MS, limit of detection 190 amol/µl injected solution).EG, its 13C-labeled analog {13C 4 ]-EG and 3,5-PFB 2 -EG were synthesized and characterized by UV and fluorescence spectrophotometry, 1H- and 13C-NMR spectroscopy and mass spectrometry. The standards were used to optimize the isolation of EG and its derivatization with pentafluorobenzyl bromide (electrophore labeling) at fmol quantities. DNA solutions were spiked with EG, the DNA was depurinated by mild acid hydrolysis, and EG was isolated from the hydrolysates by low-pressure strong cation exchange chromatography with subsequent C 18 solid-phase extraction. The extracted EG was electrophore labeled and 3,5-PFB 2 EG was detected using GC-NICI-MS. [13C 4 ] EG served as internal standard. 3,5-PFB 2 -EG was quantitated relative to its 13C-labeied analog by measuring the ion ratio m/z 354/358. The limit of detection for the complete method was 60 fmol EG/µmol guanine. The method was applied to liver DNA from young Sprague-Dawley rats exposed to 600 p.p.m. VC from day 10 through day 14 after birth. The EG concentration in these samples was 1.8 ± 0.3 pmo/µmol guanine. |
| Document Type: | text |
| File Description: | text/html |
| Language: | English |
| Relation: | http://carcin.oxfordjournals.org/cgi/content/short/11/8/1279; http://dx.doi.org/10.1093/carcin/11.8.1279 |
| DOI: | 10.1093/carcin/11.8.1279 |
| Availability: | https://doi.org/10.1093/carcin/11.8.1279 http://carcin.oxfordjournals.org/cgi/content/short/11/8/1279 |
| Rights: | Copyright (C) 1990, Oxford University Press |
| Accession Number: | edsbas.CDFB75DD |
| Database: | BASE |
| Description not available. |