Academic Journal
A Stand-Alone Microfluidic Chip for Long-Term Cell Culture
| Title: | A Stand-Alone Microfluidic Chip for Long-Term Cell Culture |
|---|---|
| Authors: | Feng, Yibo, Zeng, Yang, Fu, Jiahao, Che, Bingchen, Jing, Guangyin, Liu, Yonggang, Sun, Dan, Zhang, Ce |
| Superior Title: | Micromachines (Basel) |
| Publisher Information: | MDPI |
| Publication Year: | 2023 |
| Collection: | PubMed Central (PMC) |
| Subject Terms: | Article |
| Description: | Live-cell microscopy is crucial for biomedical studies and clinical tests. The technique is, however, limited to few laboratories due to its high cost and bulky size of the necessary culture equipment. In this study, we propose a portable microfluidic-cell-culture system, which is merely [Formula: see text] in dimension, powered by a conventional alkali battery and costs less than USD 20. For long-term cell culture, a fresh culture medium exposed to 5% CO(2) is programmed to be delivered to the culture chamber at defined time intervals. The 37 [Formula: see text] culture temperature is maintained by timely electrifying the ITO glass slide underneath the culture chamber. Our results demonstrate that 3T3 fibroblasts, HepG2 cells, MB-231 cells and tumor spheroids can be well-maintained for more than 48 h on top of the microscope stage and show physical characters (e.g., morphology and mobility) and growth rate on par with the commercial stage-top incubator and the widely adopted CO(2) incubator. The proposed portable cell culture device is, therefore, suitable for simple live-cell studies in the lab and cell experiments in the field when samples cannot be shipped. |
| Document Type: | text |
| Language: | English |
| Relation: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863834/; http://www.ncbi.nlm.nih.gov/pubmed/36677268; http://dx.doi.org/10.3390/mi14010207 |
| DOI: | 10.3390/mi14010207 |
| Availability: | https://doi.org/10.3390/mi14010207 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863834/ http://www.ncbi.nlm.nih.gov/pubmed/36677268 |
| Rights: | © 2023 by the authors. ; https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| Accession Number: | edsbas.A162B5E0 |
| Database: | BASE |
| Description not available. |