Academic Journal
Isolation of cellotriosyl blocks from barley β-glucan with endo -1,4-β-glucanase from Trichoderma reesei
| Title: | Isolation of cellotriosyl blocks from barley β-glucan with endo -1,4-β-glucanase from Trichoderma reesei |
|---|---|
| Authors: | Ajithkumar, Anu, Andersson, Roger, Siika-aho, Matti, Tenkanen, Maija, Åman, Per |
| Superior Title: | Ajithkumar , A , Andersson , R , Siika-aho , M , Tenkanen , M & Åman , P 2006 , ' Isolation of cellotriosyl blocks from barley β-glucan with endo -1,4-β-glucanase from Trichoderma reesei ' , Carbohydrate Polymers , vol. 64 , no. 2 , pp. 233-238 . https://doi.org/10.1016/j.carbpol.2005.11.033 |
| Publication Year: | 2006 |
| Subject Terms: | Barley beta-glucan, Cellotriosyl blocks, Endo-glucanase |
| Description: | Mixed-linkage β-glucan from barley, with a cellotriosyl to cellotetraosyl ratio of 2.9, was hydrolysed with two endo-1,4-β-glucanases (cellulases) and one non-cellulolytic β-glucanase isolated from Trichoderma reesei . The hydrolysates were precipitated in 90% ethanol and the fragments obtained were further treated with lichenase, followed by analysis of the oligosaccharides released. One of the endo-1,4-β glucanases, Cel 5A (EG II), selectively degraded cellotetraosyl units in the polymer and left the cellotriosyl units unhydrolysed. Blocks of cellotriosyl units were isolated on a larger scale using this enzyme. The isolated blocks were fractionated into four fractions using gel permeation chromatography on Biogel P-6 and the structures of the blocks were analysed by 1H NMR spectroscopy. The fractions essentially contained cellotriosyl units of different sizes with a 3-linked glucose residue at the non-reducing end and a reducing end linked to two 4-linked glucose residues. The results thus indicated that the enzyme could hydrolyse a 4-linked glucose residue next to the 3-linked residue at the non-reducing terminal but left two 4-linked glucose residues at the reducing end. The isolated blocks of cellotriosyl units had a molecular weight distribution that fits a theoretical model based on random blocks of triosyl units in the mixed-linkage β-glucan. |
| Document Type: | article in journal/newspaper |
| Language: | English |
| DOI: | 10.1016/j.carbpol.2005.11.033 |
| Availability: | https://doi.org/10.1016/j.carbpol.2005.11.033 https://cris.vtt.fi/en/publications/ed40efbc-55f9-4617-bca2-dd84d2d27d28 |
| Rights: | info:eu-repo/semantics/closedAccess |
| Accession Number: | edsbas.5F00A84C |
| Database: | BASE |
| Description not available. |