Academic Journal
One-step method for isolation and purification of native beta-lactoglobulin from bovine whey
| Title: | One-step method for isolation and purification of native beta-lactoglobulin from bovine whey |
|---|---|
| Authors: | Stojadinović, Marija M., Burazer, Lidija M., Ercili-Cura, Dilek, Sancho, Ana, Buchert, Johanna, Ćirković-Veličković, Tanja, Stanić-Vučinić, Dragana |
| Superior Title: | Journal of the Science of Food and Agriculture |
| Publisher Information: | Wiley-Blackwell, Malden |
| Publication Year: | 2012 |
| Collection: | CHERRY - CHEMistry RepositorY, Faculty of Chemistry, University of Belgrade / Repozitorijum Hemijskog fakulteta - Cherry (Univerzitet u Beogradu - Hemijski fakultet) |
| Subject Terms: | native ss-lactoglobulin, isolation, anion exchange chromatography, purification |
| Description: | BACKGROUND: The major whey protein beta-lactoglobulin (BLG) has been widely studied for its functional properties. The aim of this study was to develop an efficient, inexpensive and rapid one-step method for the isolation and purification of BLG while preserving its native structure. RESULTS: BLGwas purified fromdefattedwheyobtainedfromrawcow's milkbyanionexchangechromatography. Protein purity and identitywere determined using reverse phase high-performance liquid chromatography andmass spectrometry. Total BLG yield was 80% with protein purity from 97 to 99%. BLG isoforms A and B were separated into fractions of 91 and 99% purity respectively. The structure and native conformation of the isolated BLGwere compared with those of standard commercial BLG by circular dichroism spectrometry, susceptibility to various crosslinking enzymes and enzyme-linked immunosorbent assay inhibition. CONCLUSION: Theproposedmethodis veryuseful for the rapid preparationofBLGsuitable for studying antigenicandmolecular characteristics of this protein, aswell as the effect of food processing on these properties. The procedure requires only 1 day for the purification of about 300 mgof BLG from a single run using a small column (2.5 cmx20 cm) of diethylaminoethyl Sephadex and has potential for scaling up. (C) 2011 Society of Chemical Industry ; This is the peer-reviewed version of the article: (1) Stojadinović, M. M.; Burazer, L. M.; Ercili-Cura, D.; Sancho, A.; Buchert, J.; Ćirković-Veličković, T.; Stanić-Vučinić, D. One-Step Method for Isolation and Purification of Native Beta-Lactoglobulin from Bovine Whey. Journal of the Science of Food and Agriculture 2012, 92 (7), 1432–1440. [https://doi.org/10.1002/jsfa.4722]. ; Published version: [https://cherry.chem.bg.ac.rs/handle/123456789/1275] |
| Document Type: | article in journal/newspaper |
| Language: | unknown |
| ISSN: | 0022-5142 |
| Relation: | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172024/RS//; info:eu-repo/grantAgreement/EC/FP7/256716/EU//; https://cherry.chem.bg.ac.rs/handle/123456789/1275; https://doi.org/10.1002/jsfa.4722; http://cherry.chem.bg.ac.rs/handle/123456789/5261; 000302468200015; 2-s2.0-84859419583; http://cherry.chem.bg.ac.rs/bitstream/id/30278/One-step_method_acc_2012.pdf |
| DOI: | 10.1002/jsfa.4722 |
| Availability: | https://doi.org/10.1002/jsfa.4722 https://cherry.chem.bg.ac.rs/handle/123456789/1275 http://cherry.chem.bg.ac.rs/handle/123456789/5261 http://cherry.chem.bg.ac.rs/bitstream/id/30278/One-step_method_acc_2012.pdf |
| Rights: | embargoedAccess ; https://creativecommons.org/licenses/by-nc-nd/4.0/ ; BY-NC-ND |
| Accession Number: | edsbas.585BFC9E |
| Database: | BASE |
| Description not available. |