Academic Journal
Gelatin nanoparticles with tunable mechanical properties: effect of crosslinking time and loading
| Title: | Gelatin nanoparticles with tunable mechanical properties: effect of crosslinking time and loading |
|---|---|
| Authors: | Weiss, Agnes-Valencia, Schorr, Daniel, Metz, Julia K, Yildirim, Metin, Khan, Saeed Ahmad, Schneider, Marc |
| Superior Title: | Beilstein J Nanotechnol |
| Publisher Information: | Beilstein-Institut |
| Publication Year: | 2022 |
| Collection: | PubMed Central (PMC) |
| Subject Terms: | Full Research Paper |
| Description: | Tuning the elastic properties of nanoparticles intended to be used in drug delivery is of great interest. To this end, different potential formulations are developed since the particle elasticity is affecting the in vitro and in vivo performance of the nanoparticles. Here we present a method to determine the elasticity of single gelatin nanoparticles (GNPs). Furthermore, we introduce the possibility of tuning the elastic properties of gelatin nanoparticles during their preparation through crosslinking time. Young’s moduli from 5.48 to 14.26 MPa have been obtained. Additionally, the possibility to measure the elasticity of single nanoparticles revealed the influence of loading a macromolecular model drug (FITC-dextran) on the mechanical properties, which decreased with raising amounts of loaded drug. Loaded particles were significantly softer, with Young’s moduli between 1.06 and 5.79 MPa for the same crosslinking time, than the blank GNPs. In contrast to this, lysozyme as a crosslinkable macromolecule did not influence the mechanical properties. A good in vitro cell compatibility was found investigating blank GNPs and FITC-dextran-loaded GNPs in viability assays with the cancer cell line A549 and the human primary cell-derived hAELVi cell line. |
| Document Type: | text |
| Language: | English |
| Relation: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9443426/; http://www.ncbi.nlm.nih.gov/pubmed/36105690; http://dx.doi.org/10.3762/bjnano.13.68 |
| DOI: | 10.3762/bjnano.13.68 |
| Availability: | https://doi.org/10.3762/bjnano.13.68 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9443426/ http://www.ncbi.nlm.nih.gov/pubmed/36105690 |
| Rights: | Copyright © 2022, Weiss et al. ; https://creativecommons.org/licenses/by/4.0/This is an open access article licensed under the terms of the Beilstein-Institut Open Access License Agreement (https://www.beilstein-journals.org/bjnano/terms/terms), which is identical to the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ). The reuse of material under this license requires that the author(s), source and license are credited. Third-party material in this article could be subject to other licenses (typically indicated in the credit line), and in this case, users are required to obtain permission from the license holder to reuse the material. |
| Accession Number: | edsbas.526F8FF2 |
| Database: | BASE |
| Description not available. |