Bibliographic Details
| Title: |
Characterization of a Bacillus thuringiensis chitinase that binds to cellulose and chitin. |
| Authors: |
Honda, Shotaro, Kunii, Toshiyuki, Nohara, Kenta, Wakita, Satoshi, Sugahara, Yasusato, Kawakita, Masao, Oyama, Fumitaka, Sakaguchi, Masayoshi |
| Superior Title: |
AMB Express; 2/28/2017, Vol. 7 Issue 1, p1-11, 11p |
| Subject Terms: |
BACILLUS thuringiensis, CHITINASE, CELLULOSE, CHITIN, BIOPESTICIDES, LISTERIA |
| Abstract: |
Bacillus thuringiensis is a Gram-positive soil bacterium that is known to be a bacterial biopesticide that produces insecticidal proteins called crystal proteins (Cry). In the insecticidal process, chitinases are suggested to perforate the peritrophic membrane barrier to facilitate the invasion of the Cry proteins into epithelial membranes. A chitinase gene from B. thuringiensis was successfully expressed in a soluble form in Escherichia coli, and the gene product was purified and characterized. The purified recombinant enzyme, BthChi74, hydrolyzed an artificial substrate, 4-nitrophenyl N, N′-diacetyl-β- d-chitobioside [4NP-(GlcNAc)], and the natural substrates, colloidal chitin and crystalline α-chitin, but it did not hydrolyze cellulose. BthChi74 exhibited catalytic activity under a weakly acidic to neutral pH range at 50 °C, and it was stable over a wide pH range for 24 h. Differential scanning fluorimetry (DSF) indicated a protein melting temperature ( T ) of 63.6 °C. Kinetic analysis revealed k and K values of 1.5 s and 159 μM, respectively, with 4NP-(GlcNAc) as a substrate. BthChi74 produced (GlcNAc) and GlcNAc from colloidal chitin and α-chitin as substrates, but the activity toward the latter was lower than that toward the former. BthChi74 could bind similarly to chitin beads, crystalline α-chitin, and cellulose through a unique family 2 carbohydrate-binding module (CBM2). The structure-function relationships of BthChi74 are discussed in relation to other chitinases, such as Listeria chitinase, which possesses a family 5 carbohydrate-binding module (CBM5). [ABSTRACT FROM AUTHOR] |
|
Copyright of AMB Express is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
