Bibliographic Details
| Title: |
Assessment of endoglucanase activity by analyzing the degree of cellulose polymerization and high-throughput analysis by near-infrared spectroscopy. |
| Authors: |
Horikawa, Yoshiki, Imai, Tomoya, Abe, Kentaro, Sakakibara, Keita, Tsujii, Yoshinobu, Mihashi, Asako, Kobayashi, Yoshinori, Sugiyama, Junji |
| Superior Title: |
Cellulose; Jun2016, Vol. 23 Issue 3, p1565-1572, 8p |
| Subject Terms: |
CELLULOSE, GLUCANASES, POLYMERIZATION research, EUCALYPTUS globulus, TRICHODERMA reesei, HYDROLYSIS |
| Abstract: |
We developed a novel and practical assessment technique for endoglucanase (EG) activity by measuring the degree of polymerization (DP) of cellulose from Eucalyptus globulus. This evaluation method demonstrated that EG II from Trichoderma reesei had higher endoglucanase activity than EG I, which has not been detected in conventional experiments using carboxymethyl cellulose as a model substrate. In addition, a high-throughput protocol for DP measurement was developed by using near-infrared spectroscopy combined with a multivariate analysis. Interpreting the regression coefficient, a reciprocal correlation was observed between the relative crystallinity of the cellulosic residue after enzymatic hydrolysis and the DP. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
