Bibliographic Details
| Title: |
Action of fungal β-glucosidase on the mono-O-methylated p-nitrophenyl β-d-glucopyranoside 2ndICC 2007, Tokyo, Japan, October 25–29, 2007. |
| Authors: |
Takeshi Nishimura1, Mitsuro Ishihara2 |
| Superior Title: |
Holzforschung: International Journal of the Biology, Chemistry, Physics, & Technology of Wood. Jan2009, Vol. 63 Issue 1, p47-51. 5p. 1 Diagram, 2 Charts, 2 Graphs. |
| Subject Terms: |
*GLUCOSIDASES, *ENZYME kinetics, *FUNGAL enzymes, *MOLECULAR recognition, *MOLECULAR structure, *METHYL groups, *REARRANGEMENTS (Chemistry) |
| Abstract: |
AbstractThe action of β-glucosidase on the methyl derivatives of p-nitrophenyl β-d-glucopyranoside (pNP-β-Glc), which were regio-specifically substituted at O-2, O-3, O-4, and O-6positions, was studied. Specifically, several β-glucosidases isolated from brown-rot, white-rot, soft-rot fungi, and almond were investigated. These β-glucosidases did not act on the 2, 3, and 4-O-methyl derivatives, while the 6-O-methyl one was hydrolyzed by all the enzymes to some extent. The results indicate that the methyl group at O-2, O-3, and O-4of the glucopyranoside strongly inhibits the recognition by the β-glucopyranoside, while the enzymes do not discriminate the structure difference between pNP-β-Glc and its methyl derivative at O-6. [ABSTRACT FROM AUTHOR] |
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| Database: |
Academic Search Premier |
