Bibliographic Details
| Title: |
Glucans with Different Degrees of Polymerization from Leuconostoc mesenteroides CICC6055: Analysis of Physicochemical Properties and Intestinal Prebiotic Function. |
| Authors: |
Gu, Jiabao1 (AUTHOR) gjb9959228@163.com, Jiao, Ziyan1 (AUTHOR), Wang, Tao1 (AUTHOR), Zhang, Bolin1 (AUTHOR), Zhao, Hongfei1 (AUTHOR) zhaohf518@bjfu.edu.cn |
| Superior Title: |
International Journal of Molecular Sciences. Jan2024, Vol. 25 Issue 1, p258. 19p. |
| Subject Terms: |
*LEUCONOSTOC mesenteroides, *DEGREE of polymerization, *NUCLEAR magnetic resonance spectroscopy, *BETA-glucans, *HIGH performance liquid chromatography, *SHORT-chain fatty acids, *THIN layer chromatography, *GLUCANS |
| Abstract: |
This study explored the physicochemical properties and prebiotic activities of glucans and oligoglucans. Oligoglucans were obtained through the fermentation of Leuconostoc mesenteroides CICC6055 and the glucansucrase of strain CICC6055, while glucans were obtained only through fermentation. Thin-layer chromatography and high-performance liquid chromatography identified enzymatically synthesized oligoglucans with a higher yield. Differential scanning calorimetry and derivative thermogravimetry analyses revealed the heat resistance of the glucans and oligoglucans at 280–300 °C. Fourier transform-infrared spectroscopy and nuclear magnetic resonance analyses demonstrated that their main chains were linked with α-1,6-glycosidic bonds accompanied by glucose residue branching. In vitro fermentation experiments demonstrated that they not only improved the contents of short-chain fatty acids but also raised the abundance of predominant flora, such as Bacteroides, Firmicutes, Verrucomicrobia, and Proteobacteria. These results implicate glucansucrase as an efficacious tool for the enzyme synthesis of oligoglucans. Furthermore, both polysaccharides with different degrees of polymerization may be beneficial in maintaining a healthy human gut. [ABSTRACT FROM AUTHOR] |
|
Copyright of International Journal of Molecular Sciences is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Academic Search Premier |
