Bibliographic Details
| Title: |
A novel class of adhesion acidic glycans in sea urchin embryos. |
| Authors: |
Papakonstantinou, Eleni1, Karakjulakis, George2, Aletras, Alexios J.3, Misevic, Gradimir N.3 |
| Superior Title: |
European Journal of Biochemistry. 9/15/94, Vol. 224 Issue 3, p1067-1077. 11p. |
| Subject Terms: |
*SEA urchin embryos, *GEL permeation chromatography, *CELL adhesion, *ACIDS, *MESENCHYME, *MONOCLONAL antibodies |
| Abstract: |
Total glycans were isolated and purified form Lytechinus pictus embryos at early developmental stages by gel-filtration chromatography after pronase and Dnase digestion, and alkali-borohydride treatment. Fractionation by Superose 6 and HPLC gel-filtration chromatography revealed three major glycan fraction of 580, 150 and kDa consistently throughout development up to the stage of end gastrula. The 580-kDa and the 150-kDa glycan fractions have no detectable uronic acid residues and charged groups. Chemical analysis of the glycan fractions showed that their content of neutral hexoses, uronic acid, GlcNAc, GalNAc and sulphate changes during development. The resistance of the 580-kDa and the 150-kDa ad the 2-kDa glycan fractions showed that glycan synthesis increases in a linear fashion from the stage of early blastulation to end of gasturalation. Maximal incorporation of the radioligad occurs in the 2-kDa glycan fractions, with the highest rate observed between the stages of mesenchyme blastula and early gastrulation. Immunological studies , using a monoclonal antibody which inhibits cell aggregation and the end of gastrulation carry cell-adhesion epitopes. The number of these epitopes, as indicated by the intensity of the immunostaining, increases from morula formation to end-gastrulation stages and correlates with the increased rate morphogenetic movements. These results suggest tat controlled expression of the cell-adhesion glycan epitopes play an important role in sea urchin gastrulation. [ABSTRACT FROM AUTHOR] |
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| Database: |
Academic Search Premier |